Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
hcp-3

Cell type

Cell type Class
Embryo
Cell type
Early embryo
NA
NA

Attributes by original data submitter

Sample

source_name
Early embryos
strain
WYY35
developmental stage
Early embryos
chip antibody
SDQ0804 (Rabbit anti-HCP-3)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed as previously described (Gassmann et al., 2012) with slightly modification. Fixed embryos were suspended in 5 pellet volumes of ChIP buffer (+ protease inhibitors). 1 mL suspension was transferred to a milliTUBE (Covaris). Chromatin shearing was performed at 6-10 °C with Covaris M220 following settings: Processing Time: 8-20 min,Duty Cycle: 10%,Intensity: 75 Watts, Cycles per Burst: 200. The chromatin fragmentation was assessed by the smear pattern on 1% agarose gel, which was found to be between 200 and 500 bp. About 3 mg protein was diluted to 900 ml with ChIP buffer with 1 % Sarcosyl, 0.1 % Na-deoxycholate and 1 mM PMSF. 25 ml of the embryo lysate was used for the preparation of input DNA. 5 ml of antibodies was added (rabbit anti-HCP3, Novus Biologicals Q0804,1 mg/ml) to the extract and rotated gently at 4 °C overnight. 50 ml of dynabeads were added afterward and rotated at 4 °C for 2 hrs. Beads washing was performed using 2 X 1 ml FA buffer for 5 min, 1 ml FA-1000 for 10 min and 1 ml FA-500 buffer, successively. Beads were resuspended in 1 ml FA-500 buffer and transferred into new 1.5-ml Eppendorf tube, followed by 10 min FA-500 buffer washing, 10 min TEL buffer washing and brief washing by TE buffer. All washing steps were performed at 4 °C. Then, 50 ml of Elution buffer was added and incubated at 67 °C for 15 min. Dynabeads were separated from the eluted protein-DNA complex by a magnetic rack. The elutes were bought up to 150 ml by Elution buffer, followed by proteinase K digestion and reverse cross-linking at 65°C overnight. Both input DNA and ChIP-ed DNA were purified by ChIP DNA Clean & Concentrator Kit (Zymo research). Library preparation and Illumina sequencing (Pair-End sequencing of 101 bp) were performed at the University of Hong Kong, Centre for Genomic Sciences (HKU, CGS). The 4 libraries (two replicates of Input and ChIP-ed DNA) were prepared based on the protocol of KAPA Hyper Prep Kit (KR0961 – v5.16). For each library, 0.8 ng of DNA was performed with reactions of end-repair, 3' end A-tailing, and indexed adaptor ligation, followed by 16 cycles of PCR amplification reaction for library enrichment. After AMPure beads purification, each library was validated by Agilent Bioanalyzer, Qubit and qPCR for quality control analysis. The library was denatured and diluted to optimal concentration and applied in the cluster generation steps. HiSeq PE Cluster Kit v4 with cBot was used for cluster generation on the flow-cell. Illumina HiSeq SBS Kit v4 was used for Pair-End 101bp sequencing that runs on HiSeq 1500.

Sequencing Platform

instrument_model
Illumina HiSeq 1500

ce11

Number of total reads
20228470
Reads aligned (%)
91.2
Duplicates removed (%)
2.0
Number of peaks
423 (qval < 1E-05)

ce10

Number of total reads
20228470
Reads aligned (%)
91.2
Duplicates removed (%)
2.0
Number of peaks
422 (qval < 1E-05)

Base call quality data from DBCLS SRA